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AS-IV targets the overexpression of miR-362-3p and negatively regulates the activity of its downstream transcription factor RUNX1 in rat lung tissue and alveolar epithelial cells to alleviate the inhibitory effect of PM2.5 on the proliferation of rat alveolar epithelial cells L2 and its driving effect on LDH release

doi: 10.1038/s41591-018-0336-8 283 YinY

There were several limitations of our work, the main one being that this was an in vitro study using human cell lines in 2D cell cultures

Oligoclonal cell lines producing high levels of biologically active RBD were selected and cloned by limiting dilution

However, PPAR is the main regulator of lipid homeostasis in the liver and was proposed as the sexually dimorphic target in MASLD